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aav gfp plasmid  (Addgene inc)


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    Addgene inc aav gfp plasmid
    Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with <t>AAV-FLEX-GFP-FLAG.</t> Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.
    Aav Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aav gfp plasmid/product/Addgene inc
    Average 95 stars, based on 63 article reviews
    aav gfp plasmid - by Bioz Stars, 2026-04
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    Images

    1) Product Images from "Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytes"

    Article Title: Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytes

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2025.104317

    Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with AAV-FLEX-GFP-FLAG. Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.
    Figure Legend Snippet: Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with AAV-FLEX-GFP-FLAG. Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.

    Techniques Used: Immunofluorescence, Transduction, Labeling



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    Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with <t>AAV-FLEX-GFP-FLAG.</t> Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.
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    Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with <t>AAV-FLEX-GFP-FLAG.</t> Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.
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    Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with <t>AAV-FLEX-GFP-FLAG.</t> Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.
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    Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with <t>AAV-FLEX-GFP-FLAG.</t> Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.
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    GPC6 overexpression activates Wnt signaling and drives NPC, NCT, and TDP-43 abnormalities. ( a , c–n ) Representative images of immunostained cortical sections of <t>AAV-transduced</t> <t>WT;</t> <t>WNT-GFP-MYC</t> animals 10 days post-injection. Dashed lines highlight the nuclei of cells transduced with AAV HA-GFP or AAV HA-GPC6 in ( a ) and ( k–n ); dashed lines outline the cell body of transduced cells in ( c–f ); arrows highlight transduced cells in ( g–j ). Scale bar: ( a , c-n ) 10 μm. ( b ) Graph quantifying nuclear MYC immunofluorescence intensity in neurons expressing HA-GFP or HA-GPC6. **** p < 0.0001, Welch’s t-test, N = 3 animals, n = 49 HA-GFP, 47 HA-GPC6-expressing cells. ( o–r ) Graphs quantifying neurons expression HA-GFP-MYC or HA-GPC6 comparing ( o ) Nup98 C/Nuclear envelope (NE) ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 78 HA-GFP-MYC, 77 HA-GPC6-expressing cells.), ( p ) Ran N/C ratio (**** p < 0.0001,Welch’s t-test, N = 3 animals, n = 79 HA-GFP-MYC, 76 HA-GPC6-expressing cells.), ( q ) TDP-43 N/C ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.), ( r ) TDP-43 nuclear intensity (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.). A.U.: arbitrary unit. All graphs: mean ± sem.
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    GPC6 overexpression activates Wnt signaling and drives NPC, NCT, and TDP-43 abnormalities. ( a , c–n ) Representative images of immunostained cortical sections of <t>AAV-transduced</t> <t>WT;</t> <t>WNT-GFP-MYC</t> animals 10 days post-injection. Dashed lines highlight the nuclei of cells transduced with AAV HA-GFP or AAV HA-GPC6 in ( a ) and ( k–n ); dashed lines outline the cell body of transduced cells in ( c–f ); arrows highlight transduced cells in ( g–j ). Scale bar: ( a , c-n ) 10 μm. ( b ) Graph quantifying nuclear MYC immunofluorescence intensity in neurons expressing HA-GFP or HA-GPC6. **** p < 0.0001, Welch’s t-test, N = 3 animals, n = 49 HA-GFP, 47 HA-GPC6-expressing cells. ( o–r ) Graphs quantifying neurons expression HA-GFP-MYC or HA-GPC6 comparing ( o ) Nup98 C/Nuclear envelope (NE) ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 78 HA-GFP-MYC, 77 HA-GPC6-expressing cells.), ( p ) Ran N/C ratio (**** p < 0.0001,Welch’s t-test, N = 3 animals, n = 79 HA-GFP-MYC, 76 HA-GPC6-expressing cells.), ( q ) TDP-43 N/C ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.), ( r ) TDP-43 nuclear intensity (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.). A.U.: arbitrary unit. All graphs: mean ± sem.
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    aav vectors  (Addgene inc)
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    GPC6 overexpression activates Wnt signaling and drives NPC, NCT, and TDP-43 abnormalities. ( a , c–n ) Representative images of immunostained cortical sections of <t>AAV-transduced</t> <t>WT;</t> <t>WNT-GFP-MYC</t> animals 10 days post-injection. Dashed lines highlight the nuclei of cells transduced with AAV HA-GFP or AAV HA-GPC6 in ( a ) and ( k–n ); dashed lines outline the cell body of transduced cells in ( c–f ); arrows highlight transduced cells in ( g–j ). Scale bar: ( a , c-n ) 10 μm. ( b ) Graph quantifying nuclear MYC immunofluorescence intensity in neurons expressing HA-GFP or HA-GPC6. **** p < 0.0001, Welch’s t-test, N = 3 animals, n = 49 HA-GFP, 47 HA-GPC6-expressing cells. ( o–r ) Graphs quantifying neurons expression HA-GFP-MYC or HA-GPC6 comparing ( o ) Nup98 C/Nuclear envelope (NE) ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 78 HA-GFP-MYC, 77 HA-GPC6-expressing cells.), ( p ) Ran N/C ratio (**** p < 0.0001,Welch’s t-test, N = 3 animals, n = 79 HA-GFP-MYC, 76 HA-GPC6-expressing cells.), ( q ) TDP-43 N/C ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.), ( r ) TDP-43 nuclear intensity (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.). A.U.: arbitrary unit. All graphs: mean ± sem.
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    Image Search Results


    Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with AAV-FLEX-GFP-FLAG. Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.

    Journal: STAR Protocols

    Article Title: Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytes

    doi: 10.1016/j.xpro.2025.104317

    Figure Lengend Snippet: Assessment of Futile Creatine Cycle within mature brown adipocytes (A) Representative immunofluorescence images of iBAT transduced with AAV-FLEX-GFP-FLAG. Mature adipocytes were labelled with anti-Perilipin 1 (PLIN1) antibody (red), GFP-FLAG was labeled with anti-FLAG antibody (green). Nuclei were labelled with DAPI (blue). Scale bar, 100 μm. Figure adapted from figure 1d of Bunk et al. (B) Oxygen consumption rates basally and following sequential additions of noradrenaline (NA, 0.1 μM) and oligomycin (Oligo, 15 μM). SBI-425 (10 μM) was added at the start (n = 2 independent preparations/sex). Figure adapted from figure 5b of Bunk et al. Two-way ANOVA (Tukey’s post-hoc test). Data are represented as mean ± SEM.

    Article Snippet: AAV GFP (plasmid) , Addgene , 49055.

    Techniques: Immunofluorescence, Transduction, Labeling

    GPC6 overexpression activates Wnt signaling and drives NPC, NCT, and TDP-43 abnormalities. ( a , c–n ) Representative images of immunostained cortical sections of AAV-transduced WT; WNT-GFP-MYC animals 10 days post-injection. Dashed lines highlight the nuclei of cells transduced with AAV HA-GFP or AAV HA-GPC6 in ( a ) and ( k–n ); dashed lines outline the cell body of transduced cells in ( c–f ); arrows highlight transduced cells in ( g–j ). Scale bar: ( a , c-n ) 10 μm. ( b ) Graph quantifying nuclear MYC immunofluorescence intensity in neurons expressing HA-GFP or HA-GPC6. **** p < 0.0001, Welch’s t-test, N = 3 animals, n = 49 HA-GFP, 47 HA-GPC6-expressing cells. ( o–r ) Graphs quantifying neurons expression HA-GFP-MYC or HA-GPC6 comparing ( o ) Nup98 C/Nuclear envelope (NE) ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 78 HA-GFP-MYC, 77 HA-GPC6-expressing cells.), ( p ) Ran N/C ratio (**** p < 0.0001,Welch’s t-test, N = 3 animals, n = 79 HA-GFP-MYC, 76 HA-GPC6-expressing cells.), ( q ) TDP-43 N/C ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.), ( r ) TDP-43 nuclear intensity (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.). A.U.: arbitrary unit. All graphs: mean ± sem.

    Journal: Scientific Reports

    Article Title: Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons

    doi: 10.1038/s41598-025-32069-9

    Figure Lengend Snippet: GPC6 overexpression activates Wnt signaling and drives NPC, NCT, and TDP-43 abnormalities. ( a , c–n ) Representative images of immunostained cortical sections of AAV-transduced WT; WNT-GFP-MYC animals 10 days post-injection. Dashed lines highlight the nuclei of cells transduced with AAV HA-GFP or AAV HA-GPC6 in ( a ) and ( k–n ); dashed lines outline the cell body of transduced cells in ( c–f ); arrows highlight transduced cells in ( g–j ). Scale bar: ( a , c-n ) 10 μm. ( b ) Graph quantifying nuclear MYC immunofluorescence intensity in neurons expressing HA-GFP or HA-GPC6. **** p < 0.0001, Welch’s t-test, N = 3 animals, n = 49 HA-GFP, 47 HA-GPC6-expressing cells. ( o–r ) Graphs quantifying neurons expression HA-GFP-MYC or HA-GPC6 comparing ( o ) Nup98 C/Nuclear envelope (NE) ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 78 HA-GFP-MYC, 77 HA-GPC6-expressing cells.), ( p ) Ran N/C ratio (**** p < 0.0001,Welch’s t-test, N = 3 animals, n = 79 HA-GFP-MYC, 76 HA-GPC6-expressing cells.), ( q ) TDP-43 N/C ratio (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.), ( r ) TDP-43 nuclear intensity (**** p < 0.0001, Welch’s t-test, N = 3 animals, n = 67 HA-GFP-MYC, 62 HA-GPC6-expressing cells.). A.U.: arbitrary unit. All graphs: mean ± sem.

    Article Snippet: All AAV plasmid backbones were based on AAV-GFP.Cre (Addgene, 49056).

    Techniques: Over Expression, Injection, Transduction, Immunofluorescence, Expressing